[Research Interests]

Sexual Reproduction in flowering plants is a process of higher complexity than that in animals. In angiosperms, a pollen mother cell undergoes meiosis to generate four microspores. Each microspore then undergoes the first haploid mitosis (microspore mitosis), which is a typical example of asymmetrical mitosis, giving rise to a large vegetative cell and a small generative cell. Soon the generative cell migrates into the cytoplasm of the vegetative cell. The generative cell finally divides into two sperm cells.
The process described above is called "pollen development". The developmental process of lily pollen is highly synchronized, and successive stages can easily be estimated from the length of the buds on the plant.

We are interested in chromatin configuration, chromatin dynamics and cell differentiation of pollen development.

[Key Words]
Pollen, Gamete, Meiosis, Chromatin, Histone, etc.

Except for high synchronity and capable of stage estimate from bud length, lily pollen has the great advantage of isolating the protoplast and the generative cell.

1. Lily pollen

2. Treated with cellulase and pectinase

3. Purified protoplasts

4. Generative cells isolated from pollen protoplasts

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